Western Blot analysis of ATP5H using ATP5H Polyclonal Antibody (Product # PA5-112185) at a dilution of 1:500. Lane A: HeLa whole cell lysate, Lane B: HepG2 whole cell lysate, Lane C: PC-3 whole cell lysate, Lane D: U251MG whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 20 kDa.